A team of researchers from the Faculty of Sciences of the National University of Singapore He provided evidence of cytotoxic effect on cancer cells lung black cardamomwhich has been used in Ayurvedic medicine since India treat the cancer and lung conditions.
The main problems associated with the existing drugs against lung cancer serious side effects and drug resistance. Hence the constant need to explore new molecules to improve the survival rate and quality of life of patients with lung cancer.
This new research, published in the scientific journal “Journal of Ethnopharmacology”, has shown that this spice is a source of powerful bioactivessuch as cardamon and alpinetine, which could be used in the treatment or prevention of lung cancer.
Thus, this study is the first to report the association of black cardamom extract with induction of oxidative stress in lung cancer cellsand compares the spice’s effects on lung, breast and liver cancer cells.
The research provides verification of the ethnomedical uses of black cardamom for its effect on lung conditions. Black cardamom is sIt is often used in Asian homes in rice preparations, curries and stews.in whole spice or powder form.
This spice is also prescribed in the Ayurvedic medicine in powder form, where it is used for conditions such as cough, chest congestion, pulmonary tuberculosis and throat disease. Also, black cardamom has been used in medicinal formulas for cancer patients in some rural and tribal cultures of India.
In this study, the fruits of black cardamom pulverized and extracted sequentially with five kinds of solvents, including organic solvents and water. This allowed the researchers to evaluate the best solvents to extract the most potent active ingredients from the fruit.
Then he checked the cytotoxicity of the different types of extracts of black cardamom against various types of cancer cells. It was about lung, liver and breast cancer cells. Of the three cell types, lung cells survived the least when tested with black cardamom extracts.
“The study lays the groundwork for further study whether eating black cardamom can prevent lung cancer Or serve as therapy. Previous studies on the effects of black cardamom on cancer were preliminary and did not link the research findings to the use of black cardamom in traditional medicine. There were also not enough analyzes done with different cancer cells to know which cancer cells responded best to black cardamom extracts,” says Pooja Makhija, one of the leaders of the work.
The hexane sequential extraction method followed by dichloromethane produced an extract of black cardamom that was more effective against lung cancer cells. Cells treated with dichloromethane extract mainly die through the apoptotic pathway, as the live cell measurement has been reduced to an average of about 20% after 48 hours of contact with black cardamom extracted with dichloromethane.
Cell death was caused by apoptosis with cells that showed morphological changessuch as shape deformation and shrinkage, increased oxidative stress, and failure to repair DNA damage.
After subjecting the black cardamom extract to liquid chromatography-mass spectrometry analysis, the researchers linked the presence of two well-studied bioactives, cardamonine and alpinetinewith the cytotoxic potential of black cardamom.
“Since black cardamom is commonly used as an important spice in the kitchen, further investigation into its impact on lung cancer progression in preclinical models could provide strong evidence in support of the “food as medicine” philosophy of Hippocrates, largely neglected today,” explains Gautam Sethi, another research collaborator.